Covalent Immobilization of Aldehyde and Alcohol Dehydrogenases on Ordered Mesoporous Silicas
نویسندگان
چکیده
Abstract Purpose This work studies the immobilization of two enzymes, alcohol dehydrogenase (ADH) and aldehyde (AldDH) both from Saccharomyces cerevisiae , which could be used to produce high value-added molecules carboxylic acids embedded in anaerobic digestate. Methods In particular, three mesoporous siliceous materials, with different specific surface areas pore sizes, (MSU-H, MSU-F MCF 0.75 ) were as supports for covalent immobilization. The support materials characterized by complementary techniques. Then, after a functionalization, creating bond between enzyme was performed. activity yield biocatalysts then evaluated. Results best results obtained MSU-H MSU-F, resulting an greater than 50% all cases, 0.13 IU/g supp AldDH/MSU-H, 0.10 AldDH/MSU-F, 48.6 ADH/MSU-H 12.6 ADH/MSU-H. These optimal pH temperature stability factor With ADH/MSU-F no decrease observed 120 h incubated at 50 °C. Finally, AldDH/MSU-H perform reduction reaction it seen that five cycles residual 20% cases. Conclusion ADH AldDH enzymes have been successfully immobilized on supports, considerably increasing their thermal being able reuse them several cycles. use this these is adaptable wide variety enzymes. Graphical
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ژورنال
عنوان ژورنال: Waste and Biomass Valorization
سال: 2022
ISSN: ['1877-2641', '1877-265X']
DOI: https://doi.org/10.1007/s12649-022-01812-y